The topology of DNA duplexes changes during replication and also after deproteinization in vitro. Here we describe these changes and then discuss for the first time how the distribution of superhelical stress affects the DNA topology of replication intermediates, taking into account the progression of replication forks. The high processivity of Topo IV to relax the left‐handed (+) supercoiling that transiently accumulates ahead of the forks is not essential, since DNA gyrase and swiveling of the forks cooperate with Topo IV (...) to accomplish this task in vivo. We conclude that despite Topo IV has a lower processivity to unlink the right‐handed (+) crossings of pre‐catenanes and fully replicated catenanes, this is indeed its main role in vivo. This would explain why in the absence of Topo IV replication goes‐on, but fully replicated sister duplexes remain heavily catenated. (shrink)

During replication, the topology of DNA changes continuously in response to well‐known activities of DNA helicases, polymerases, and topoisomerases. However, replisomes do not always progress at a constant speed and can slow‐down and even stall at precise sites. The way these changes in the rate of replisome progression affect DNA topology is not yet well understood. The interplay of DNA topology and replication in several cases where progression of replication forks reacts differently to changes in DNA topology ahead is discussed (...) here. It is proposed, there are at least two types of replication fork barriers: those that behave also as topological barriers and those that do not. Two‐Dimensional (2D) agarose gel electrophoresis is the method of choice to distinguish between these two different types of replication fork barriers. (shrink)

In lieu of an abstract, here is a brief excerpt of the content:Reviewed by:Critical Tales: New Studies of the Heptaméron and Early Modern CultureDora E. PolachekCritical Tales: New Studies of the Heptaméron and Early Modern Culture, edited by John D. Lyons and Mary B. McKinley; xii & 296 pp. Philadelphia: University of Pennsylvania Press, 1993, $36.95.What a difference a decade can make. In 1983 H. P. Clive’s slim Marguerite de Navarre: An Annotated Bibliography made pointedly clear the marginal position of (...) this author in the French Renaissance literary canon, and the relative obscurity of her Heptaméron (there were less than 200 entries dealing with her unfinished collection of stories that first appeared in 1558). The 500th anniversary of Marguerite’s birth in 1992 brought with it a remarkable outpouring of reflections on this neglected masterpiece. Critical Tales ranks among the best of such undertakings. Eclectic in its approach, the volume comprises fifteen articles by scholars whose analyses advance the reader’s appreciation of the Heptaméron in all “its inexhaustible complexity” (p. 279).As complex as Marguerite’s tales may be, Lyons and McKinley have done an admirable job making their volume’s contents as accessible as possible to the growing number of scholars interested in the work both as text and as important artifact in the history of the evolution of narrative. All the articles are in English (with three translated from French). Citations from the Heptaméron appear in French, but for each, the editors include the corresponding page numbers in the most accessible English translation. In addition to a selected bibliography, the volume provides a useful index.The volume’s introduction presents the dominant issues, as well as a sketch of each article’s vantage point. The editors then divide the book into three sections: “Generic Transformations and Graphic Transgressions” (with articles by Robert D. Cottrell, Hope Glidden, Marcel Tetel, Donald Stone, and Tom Conley); “Narrative Systems and Structures” (with articles by Michel Jeanneret, Cathleen M. Bauschatz, François Cornilliat and Ullrich Langer, McKinley herself, Philippe de Lajarte, and André Tournon); “Character and Community” (with articles by Daniel Russell, François Rigolot, Paula Sommers, and Edwin M. Duval). The epilogue that follows, by virtue of its length and quality, could easily be considered the sixteenth article in the collection. It shows how these heterogeneous pieces relate to issues of narrative and narrative theory.Just how radical an upheaval did the Reformation cause? The contributions bear witness to the major changes in ways of thinking and writing that played themselves out even in secular texts. The theological tensions caused by the evangelical movement are evident in all three sections of the volume, and the introduction will lead readers to the relevant essays. Among them, Rigolot’s striking allegorical reading of novella 32 in terms of “The Magadelen Controversy” (i.e., was she really one person or a conflation of three different women?) demonstrates why the discoveries of the intellectually driven philological projects of such humanists as Lefèvre d’Etaples posed such pragmatic [End Page 392] quagmires for the late medieval church. Another piece likely to cause discussion is Duval’s “Et puis, quelles nouvelles?” His lexical findings on the Renaissance meaning of nouvelle lead him to conclude that the ten characters assembled at Saint-Serrance “do no tell tales” but rather “report news” (p. 245). For Duval the evangelical resonances of the “Good News” highlight the fundamental philosophical dilemma in the discussions of the frame story characters: how to reconcile living in the world of news while still abiding to the Word of the Good News.For articles more specifically on narrative theory, readers will benefit from the epilogue by using its insights and directional signs to locate those essays particularly relevant to their interests. Among these, two focus on the interplay between the revolution in printing and narrative strategies. In “Modular Narrative and the Crisis of Interpretation,” Jeanneret shows how the short tale structure of the Heptaméron was part of an important Renaissance trend favoring creation of “modules,” defined as moveable segments of discourse that could be reassembled and put to other uses, while at the same time allowing for the intercalation of commentary. In his “The Graphics of... (shrink)

The United States is one of the most charitable nations, yet comprises some of the most materialistic citizens in the world. Interestingly, little is known about how the consumer trait of materialism, as well as the opposing moral trait of gratitude, influences charitable giving. We address this gap in the literature by theorizing and empirically testing that the effects of these consumer traits on charitable behavior can be explained by diverse motivations. We discuss the theoretical implications, along with implications for (...) charitable organizations, and offer suggestions for future research. (shrink)

Professional musicians are expected to perform at a very high level of proficiency. Many times, this high standard is associated with perfectionism, which has been shown to prompt both adaptive and maladaptive motivational dynamics and outcomes among music students. The question about how perfectionism interplays with motivational dynamics in music students is still unanswered and research within this line is scarce, especially in Latin America. In the light of Self-Determination Theory, this cross-sectional study investigated the relationship between the perceptions of (...) motivational context, basic psychological needs, perfectionism, and flourishing in University music students from Lima, Peru. We performed a path analysis testing a model in which motivational teaching styles predicted both, perfectionism and flourishing via need satisfaction and frustration. The model's fit indices were ideal [χ2 = 7.48, p = 0.300, CFI = 0.998, TLI = 0.992, RMSEA = 0.021, SRMR =0.040]. In this model, perceived autonomy supportive style predicted need satisfaction positively and need frustration negatively; perceived controlling teaching style did not predict need satisfaction nor frustration. In turn, need satisfaction positively predicted adaptive perfectionism and flourishing; whereas, need frustration predicted maladaptive perfectionism. These results shed light on the relevance of perfectionism in the psychology of higher music education students. Lastly, we highlight the importance of autonomy support in fostering adaptive high standards and flourishing in music learning. (shrink)

The authors, both child psychiatrists, discuss some of the ethical problems that arise in their practice, in relation to advice given in the British Medical Association's Handbook of Medical Ethics. They find that the main problems occur when multidisciplinary cooperation is needed. Their concern about confidentiality is shared in the papers by Kenny, Pheby and their commentators, which follow this one.

A life colonization is produced due to systemic demands that as a sign of the present-day life invades it with its logical functioning throwing moral and ethical values out either in private life or in public opinion. It is also important to point out a great increase of many pathologies in private..

The proposal of the work centers on pointing out some of the relationships between the concepts that refer to the processes of socialization and subjectivation in its articulation with the understanding. For this end, a brief reference to them is made from a multidisciplinary and transversal approac..

Artificial intelligence is a general-purpose technology (GPT), term given to technologies that shape an entire era and reorient innovations by reconfiguring the economy’s logic and functioning and bringing in new business models. AI offers unprecedented opportunities and risks. The benefits of AI are extraordinary, as are its potential harms. Potential damage does not have the same degree of problematization, since the intensity and extent of the damage varies according to the domain and the object of application. To address the scale (...) of this challenge, regulation is necessary but not sufficient. Standards, unwritten codes of compliance and arbitration procedures, supervision and auditability, AI governance, international agreements, compliance with current local and global standards and laws. All of this needs to be integrated. Society seems to have no alternative to facing the challenges of at least mitigating the damage already identified and trying to predict future damage in advance. The purpose of this article is to encourage reflection regarding the main initiatives that are available to society to protect its citizens and organizations from the potential harm caused by AI models, vis-à-vis the technology’s own limits to act in ethical and legal compliance. (shrink)

In this piece, I propose a reading of Plato’s Gorgias that pays special attention to the role that the fictional audience plays in the unfolding of the dialogue. To this end, I use some of the insights that Chaïm Perelman and Lucie Olbrechts–Tyteca conveyed in their seminal work, The New Rhetoric: A Treatise on Argumentation in order to argue that thinking about the way in which Socrates’ arguments are shaped by the different audiences that Gorgias, Polus, and Callicles aim to (...) address and represent provides us with a new hermeneutical understanding of what is at stake in each of the different interactions Socrates engages in throughout the dialogue. In unpacking the way in which Socrates appropriates Gorgias’ particular audience, transforms Polus’ universal audience, and challenges Callicles’ elite audience, I provide an outline of the difficulties that Plato’s Socrates has to overcome in order to achieve the ‘community of minds’ that Perelman and Olbrechts–Tyteca identify as the bedrock of fruitful argumentation. Having done this, in the last section I turn to Plato’s Phaedrus, for the purpose of making evident that thinking about Plato’s deployment of rhetorical audiences is a crucial step in the effort to expose the implicit continuity that links the discussion of rhetoric delivered by the Gorgias to that of the Phaedrus. (shrink)

Populistic political discourse often portrays ethnic minorities as threats to the majority society. However, the deeper characteristics of perceived threats have not been sufficiently empirically investigated. The goal of this study is to identify the similarities and differences in intergroup threats perceived by Slovak majority from Roma, Muslims, and ethnic Hungarian minorities. The participants included 1244 adults who were instructed to write the first five associations that came to mind when thinking about one of the minorities. Our findings indicate that (...) power threat was dominant from the Hungarian minority and safety threat from the Roma and Muslim minorities. Moreover, the safety threat from the Roma minority related mainly to theft and violence, while from Muslims it was terrorism. Mapping and addressing specific threats associated with different minorities can help explain misperceptions and reduce prejudice against them. (shrink)

The relationship between food and religion is a lived activity formed by the dynamics of both tradition and adaption. Religious commitments to food are influenced by various factors, ranging from personal spirituality and experiences to social patterns of belonging, ethical, polit­ical and doctrinal convictions. _Challah_, _gefilte_ _fish_, _blintzes_ – these are just a few of the traditional Finnish Jewish meals that are still prepared by members of the community. The originally Eastern European dishes are one of the last living links (...) that connect assimilated Finnish Jews with their Orthodox Jewish ancestors mainly from Russia, Poland, Belarus, and Lithuania. The current paper aims to present the conceptions and reflections relating to boundaries of identity connected with the multi-ethnic culinary traditions of Jews living in Finland as well as their ways of coping with the requirements of _kashrut_. The article is based on ethnographic data from interviews as well as personal encounters, informal conversations and home visits. (shrink)

Notwithstanding universities’ many laudable aims, incidents of serious bullying, academic harassment and sexual harassment in academic settings are reported with increasing regularity globally. However, the human rights violations involved in bullying and academic harassment have not received attention by the literature. In this article, we pierce the veil of silence surrounding university environments and provide a systematic account of the breaches of international and European human rights law involved in academic bullying and harassment. By adopting a socio-legal lens, we shed (...) light onto such practices and tactics, the breaches of specific human rights and norms and the reluctance of states and regulators to intervene in what is perceived to be universities’ ‘sovereign’ sphere of jurisdiction in order to prevent human rights’ violations and to protect the victims of human rights abuse. We call for a multi-faceted and multi-agency approach to tackle academic bullying and harassment, and make a number of institutional and policy recommendations to ensure universities’ compliance with human rights standards and the effective protection of academics under attack. (shrink)

The ‘reinvention’ of nationhood in theory and the reform of British naturalization rules in praxis have been unable to address satisfactorily the issue of unjust exclusion and to make naturalization law and citizenship more compatible with democratic ideals. This has much to do with the fact that the discourse of new patriotism and the reconfiguration of national citizenship have inbuilt limits. In examining the ‘new’ discourse of patriotism in its various shades, I argue that it is inconsistent and unpersuasive. Neither (...) the rehabilitation of civic nationalism under ‘republican patriotism’, nor ‘constitutional patriotism’, nor ‘rooted patriotism’ succeed in transforming the nationality model of citizenship in order to render it more compatible with contemporary developments and with cultural pluralism. Similarly, the three models of citizenship developed by the literature, namely, postnational, transnational and multicultural citizenship remain rooted within the civic nationalist trajectory. Instead of arguing for the liberalization of naturalization requirements and the ensuing pluralization of citizenship, I put forward an argument as to how the nationality model of citizenship might be transcended by developing a model of civic registration. By contrasting this model with the Labour Government’s reforms in the fields of naturalization and citizenship, I argue that the Nationality, Immigration and Asylum Act 2002 places too much emphasis on social cohesion, thereby overlooking that a sense of belonging to community develops with inclusion in society and politics rather than as a result of citizenship ceremonies and language proficiency tests. (shrink)

v. 1. Nae Ionescu -- v. 2. Mircea Eliade -- v. 3. Octav Onicescu -- v. 4, pt. 1-2. Noica şi Securitatea.

Vaccines and vaccination are richly explored areas of study within the history of science and medicine, connecting related fields of the history of science and technology, and spanning across subfields such as biomedical sciences, animal studies, colonial and postcolonial history, and the history of global health. Vaccination is a thoroughly political act that is at once an intimate and local issue and a transnational one, with its particular set of politics connecting stakes for the individual and the community. Vaccination also (...) maps on narratives and temporal frameworks of disease with an ultimate goal of ending epidemics. Therefore, the essay takes these three analytical entry points to discuss the historiography of vaccination: the geographical, the political, and the temporal. We argue that through these lenses we can gain a more nuanced understanding of historical narratives we privilege, and in return, this understanding can enable us to explore past and current questions of health inequalities, validation practices, power relations and resistance and vaccine diplomacy. (shrink)

The framing of the debate concerning sovereignty in terms of the dualism of retention or rejection conceals the floating character of sovereignty and constrains the capacity of the state to mutate, adapt and respond adequately to the diverse and complex processes which range in, through and above it. The paper develops the idea of floating sovereignty by putting forward four main propositions: (i) sovereignty's historical entanglement with statehood makes it unsuitable for non‐state political organisations; (ii) although the state has been (...) the necessary condition for sovereignty, the latter is no longer necessary for the evolution of the state; (iii) the traditional ideological function performed by sovereignty, namely the legitimation of state power, could be performed by other organizing principles which prioritize governmental efficiency over territorial extent and democratic criteria over nationalist ones; (iv) this means that the state will no longer be in a position to command the loyalty of its citizens but it would have to purchase it through its capacity to meet social needs, to fulfil its basic functions and through the normative qualities of its institutions and policies. Three institutional designs in core areas of ‘high politics’, that is, the fields of determination of nationality, immigration policy and foreign and security policy show how floating sovereignty could be implemented. (shrink)

Whereas literature on satellites and outer space exploration has usually been dominated by vision, humankind’s initial encounter with the Earth’s first artificial satellite, Sputnik I, was overwhelmingly sonic. Tracking was originally enabled by the signal continuously transmitted by its radio beacon. Embedded in the International Geophysical Year (IGY) citizen science programs, radio amateurs played a crucial role in receiving the signal and assisting professional scientists in tracking the satellite in its initial phases. Their established existence as a distinctive worldwide community (...) of skilled operators and experimenters made hams special allies to IGY scientists and, in many respects, set them apart from other groups of amateur scientists. This essay focuses on radio amateurs’ practices and spaces of listening to Sputnik within the wider framework of the IGY. In transcending national and terrestrial boundaries, Sputnik’s signal shaped new spatialities at a variety of scales: from the macroscales of the globe and outer space to the intimate microscale of the domestic radio shack. Focusing on radio amateurs’ personal experiences and on the invisible agencies of Sputnik’s signal, the essay uses sound as a way into the sociocultural, political, and scientific importance of the IGY. More broadly, it contributes to a multisensory history (and geography) of science. (shrink)